Dynein IC2, axonemal (N105) polyclonal, anti-human

Background:
Dyneins are multi-subunit, high molecular weight ATPases that interact with microtubules to generate force by converting the chemical energy of ATP into the mechanical energy of movement. Cytoplasmic or axonemal Dynein heavy, intermediate, light and light-intermediate chains are all components of minus end-directed motors, the complex transports cellular cargos towards the central region of the cell. Axonemal Dynein motors contain one to three nonidentical heavy chains and cause a sliding of microtubules in the axonemes of cilia and flagella in a mechanism necessary for cilia to beat and propel the cell. Cytoplasmic Dynein is an approximately 12 subunit complex of two heavy chains, 2 intermediate chains to anchor Dynein to its cargo, 4 smaller intermediate chains and several light chains. It performs functions necessary for cell survival such as organelle transport and centrosome assembly. The carboxy-terminus of Dynein is important for microtubule-dependent motility and is highly conserved, while the amino-terminal regions are more variable. Several proteins regulate Dynein activity, including dynactin, LIS1 and nudel (NudE-like).

Alternative Name:
Dynein intermediate chain 2, axonemal, Axonemal dynein intermediate chain 2, DNAI2

Application Dilution:
WB: 1:500~1:1000

Specificity: Dynein IC2, axonemal (N105) polyclonal antibody detects endogenous levels of Dynein IC2, axonemal protein.

Immunogen:
Synthetic peptide, corresponding to amino acids 75-135 of Human Dynein IC2, axonemal.

MW: ~ 73kDa

Swiss-Prot: Q9GZS0

Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

For research use only, not for use in diagnostic procedure.