E-selectin (clone UZ6), anti-mouse
€564.00
In stock
SKU
ARP-21-5074
Catalog Number: 21-5074
Size: 100 µg
Isotype: Rat IgG2b
Size: 100 µg
Isotype: Rat IgG2b
Background:
Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation. This response is immediate and involves a cascade of adhesion receptors. At the endothelial surface members of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines. In vitro experiments have shown that IL-1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NF?B dependent signalling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.
Clone: UZ6
Isotype: Rat IgG2b
Source: UZ6 is a Rat monoclonal IgG2b antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a Lewis Rat immunized with LPS-activated mlEND1 cells expressing E-selectin at their cell surface.
Reactivity: mouse
Specificity: UZ6 recognizes a conformational epitope in the CR2 region in murine E-selectin. UZ6 partially inhibits leukocyte adhesion to murine activated endothelial cells.
Form: Each vial contains 100ul purified monoclonal antibody in PBS containing 0.09% sodium azide.
Applications: ELISA, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (frozen), Immunoprecipitation, Western blotting
Storage: Store at 4C, or in small aliquots at -20C.
Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation. This response is immediate and involves a cascade of adhesion receptors. At the endothelial surface members of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines. In vitro experiments have shown that IL-1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NF?B dependent signalling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.
Clone: UZ6
Isotype: Rat IgG2b
Source: UZ6 is a Rat monoclonal IgG2b antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a Lewis Rat immunized with LPS-activated mlEND1 cells expressing E-selectin at their cell surface.
Reactivity: mouse
Specificity: UZ6 recognizes a conformational epitope in the CR2 region in murine E-selectin. UZ6 partially inhibits leukocyte adhesion to murine activated endothelial cells.
Form: Each vial contains 100ul purified monoclonal antibody in PBS containing 0.09% sodium azide.
Applications: ELISA, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (frozen), Immunoprecipitation, Western blotting
Storage: Store at 4C, or in small aliquots at -20C.
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