E2F-1 (clone 4G8-2C2-C12), anti-human, rat

Background:
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.

Synonyms: E2F-1, RBAP1, RBBP3, RBP3

Cellular Location: Nucleus

Immunogen:
Purified recombinant human E2F-1 protein fragments expressed in E.coli.

Gene Symbol: E2F-1

Gene ID: 1869

Swiss prot: Q01094

Calculated MW: 70kDa

Recommended dilution:
WB 1:500, ICC 1:100,

Purity:
Affinity purification

Storage Buffer:
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:
Store at -20℃. Avoid freeze / thaw cycles.