E2F1 polyclonal, anti-human, mouse, rat

Background:
The human retinoblastoma gene product appears to play an important role in the negative regulation of cell proliferation. Functional inactivation of Rb can be mediated either through mutation or as a consequence of interaction with DNA tumor virus-encoded proteins. Of all the Rb associations described to date, the identification of a complex between Rb and the transcription factor E2F most directly implicates Rb in regulation of cell proliferation. E2F was originally identified through its role in transcriptional activation of the adenovirus E2 promoter. Sequences homologous to the E2F binding site have been found upstream of a number of genes that encode proteins with putative functions in the G1 and S phases of the cell cycle. E2F-1 is a member of a broader family of transcription regulators including E2F-2, E2F-3, E2F-4, E2F-5, E2F-6 and E2F-7 each of which forms heterodimers with a second protein, DP-1, forming an "active" E2F transcriptional regulatory complex.

Alternative Name:
E2F-1, RBAP-1, RBBP-3, Dmel\CG6376, Dmel_CG6376, drosE2F1, E(Sev-CycE)3A, E(var)3-93E, E2-promoter binding facto, E2F 1, E2F transcription factor 1, E2F-1, E2f-PA, E2f-PB, E2f-PC, E2F1, E2f1 E2F transcription factor 1, E2F1, Evar(3)164, KIAA4009, l(3)07172, l(3)j3B1, l(3)j3C2, l(3)rM729, mKIAA4009, OTTHUMP00000030661, PBR3, PRB binding protein E2F 1, PRB-binding protein E2F-1, RBAP 1, RBAP-1, RBAP1, RBBP-3, RBBP3, RBP 3, RBP3, Retinoblastoma-associated protein 1, Retinoblastoma-binding protein 3, Transcription factor E2F1,

Application Dilution:
WB:1:1,000
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:50-1:100

Specificity: E2F1 polyclonal antibody detects endogenous levels of E2F1 protein.

Immunogen:
recombinant protein

MW: 55 kDa

Swiss-Prot: Q01094(Human) Q61501(Mouse) O09139(Rat)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.