E2F2 polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
E2F2(E2F transcription factor 2) also called E2F-2, is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F2 gene is mapped to 1p36 by fluorescence in situ hybridization. Electrophoretic mobility shift assays revealed the specific binding of E2F2 to ECE1b promoter sequences containing either allele of the C-338A polymorphism, with the -338A allele being associated with an increased affinity to E2F2 compared with -338C. The ability of Myc to induce S phase was impaired in the absence of either E2f2 or E2f3 but not E2f1 or E2f4. In contrast, the ability of Myc to induce apoptosis was markedly reduced in cells deleted for E2f1 but not E2f2 or E2f3.

Description:
Rabbit IgG polyclonal antibody for Transcription factor E2F2 (E2F2) detection. Tested with WB in Human, Mouse, Rat.

Synonyms:
dE2F2 antibody, E2F transcription factor 2 antibody, E2F-2 antibody, E2F2 antibody, E2F2_HUMAN antibody, Transcription factor E2F2 antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the C-terminus of human E2F2(422-427aa ISDLFDSYDLGDLLIN), identical to the related mouse sequence.A synthetic peptide corresponding to a sequence at the C-terminus of human E2F2(422-427aa ISDLFDSYDLGDLLIN), identical to the related mouse sequence.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, rat .
Predicted to work with: mouse.

Applications: WB

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.