E2F4 polyclonal, anti-human, rat

Quantity: 100 µg

Background:
Transcription factor E2F4 is a protein that in humans is encoded by the E2F4 gene. The protein encoded by this gene is a member of the E2F family of transcription factors. This gene is mapped to 16q22.1. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins(DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer.

Description:
Rabbit IgG polyclonal antibody for Transcription factor E2F4 (E2F4) detection. Tested with WB in Human, Rat.

Synonyms:
E2F 4 antibody, E2F transcription factor 4 antibody, E2F transcription factor 4 p107/p130 binding antibody, E2F-4 antibody, E2F4 antibody, E2F4_HUMAN antibody, p107/p130 binding protein antibody, Transcription factor E2F 4 antibody, Transcription factor E2F4 antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence in the middle region of human E2F4(228-243aa LPKPALAQSQEASRPN), different from the related mouse and rat sequences by four amino acids.A synthetic peptide corresponding to a sequence in the middle region of human E2F4(228-243aa LPKPALAQSQEASRPN), different from the related mouse and rat sequences by four amino acids.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, rat

Applications: WB

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.