Early Endosome Antigen 1 (EEA1) (clone M347), anti-human, mouse, rat

Background:
Early endosomes are cellular compartments that receive endocytosed materials and sort them for vesicular transport to late endosomes and lysosomes, as well as for recycling material to the plasma membrane. Early endosome antigen 1 (EEA1) is an early endosomal protein that contains an N-terminal zinc finger motif, a cys-rich C-terminal metal-binding finger, and multiple sites for N-glycosylation, phosphorylation, and N-myristoylation. Expression of EEA1 mRNA is observed in skeletal muscle, heart, brain, lung, liver, and pancreas. Immunoblot analysis shows that EEA1 is a 180-kDa protein localized in membrane and cytosolic fractions. Immunofluorescence microscopy shows that EEA1 colocalizes with transferrin and with RAB5 in early endosomes, but not with RAB7 in late endosomes.

Immunogen: Clone M347 was generated from a recombinant protein corresponding to amino acid residues in the N-terminal region of human EEA1. This sequence has high homology to similar regions in rat and mouse EEA1.

Specificity: This antibody detects a 180 kDa* protein corresponding to the apparent molecular mass of EEA1 on SDS-PAGE immunoblots of adult mouse brain. In immunocytochemistry, anti-EEA1 specifically stains endosomal vesicles.

Buffer/Storage:
Mouse monoclonal purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.