EB3 (phospho-Ser-176) Blocking Peptide
€155.00
In stock
SKU
ECM-EX5135
Background:
Microtubles (MTs) are oriented with a fast growing plus-end and a slower growing minus-end. The MT plus-end is a crucial site for the regulation of MT dynamics and MT association with different cellular organelles by several groups of plus-end tracking proteins (+TIPs). These +TIPs form comet-like accumulations at the plus ends of MTs to regulate MT dynamics and interactions. The End-Binding (EB) family of +TIPs includes EB1 (MAPRE1), EB2 (MAPRE2, RP1), and EB3 (MAPRE3, EBF3). EB proteins are ubiquitiously expressed +TIPs that can dimerize through a coiled-coil C-terminal region, and bind MTs through an N-terminal calponin homology domain. EB proteins can stabilize MTs, increase MT dynamics, and suppress MT pauses. Site specific phosphorylation may regulate EB activity. EB3 Ser-162 phosphorylation destabilizes EB3 dimer and reduces MT growth, while aurora-kinase induced Ser-176 phosphorylation regulates EB3 protein stability during mitosis.
Sequence: Phospho-EB3 (Ser-176) synthetic peptide corresponding to amino acid residues surrounding serine 176 in human EB3. This peptide sequence is highly conserved in mouse and rat EB3. This site is conserved in EB2 but the neighboring amino acids are not well conserved.
Specificity: The peptide is specifically recognized by anti-EB3 (Ser-176) phospho-specific antibody (EP5131) in ELISA, and has been shown to block the reactivity of EP5131 in Western blot. In addition, the peptide is recommended for use in blocking EP5131 reactivity in immunocytochemistry.
Buffer/Storage:
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Microtubles (MTs) are oriented with a fast growing plus-end and a slower growing minus-end. The MT plus-end is a crucial site for the regulation of MT dynamics and MT association with different cellular organelles by several groups of plus-end tracking proteins (+TIPs). These +TIPs form comet-like accumulations at the plus ends of MTs to regulate MT dynamics and interactions. The End-Binding (EB) family of +TIPs includes EB1 (MAPRE1), EB2 (MAPRE2, RP1), and EB3 (MAPRE3, EBF3). EB proteins are ubiquitiously expressed +TIPs that can dimerize through a coiled-coil C-terminal region, and bind MTs through an N-terminal calponin homology domain. EB proteins can stabilize MTs, increase MT dynamics, and suppress MT pauses. Site specific phosphorylation may regulate EB activity. EB3 Ser-162 phosphorylation destabilizes EB3 dimer and reduces MT growth, while aurora-kinase induced Ser-176 phosphorylation regulates EB3 protein stability during mitosis.
Sequence: Phospho-EB3 (Ser-176) synthetic peptide corresponding to amino acid residues surrounding serine 176 in human EB3. This peptide sequence is highly conserved in mouse and rat EB3. This site is conserved in EB2 but the neighboring amino acids are not well conserved.
Specificity: The peptide is specifically recognized by anti-EB3 (Ser-176) phospho-specific antibody (EP5131) in ELISA, and has been shown to block the reactivity of EP5131 in Western blot. In addition, the peptide is recommended for use in blocking EP5131 reactivity in immunocytochemistry.
Buffer/Storage:
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
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