EGF human animal free recombinant protein

Background:
Recombinant human epidermal growth factor (EGF) is a 6. 3 kDa globular protein containing 54 amino acids residues, including 3 intra-molecular disulfide bonds. EGF is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. Recombinant EGF was expressed in E. coli and purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.Produced using non-animal reagents in an animal-free laboratory

Alternative Names:
Epidermal growth factor, Pro-epidermal growth factor, urogastrone, EGF

Domain: 971-1023aa
Sequence:
MNSDSECPLS HDGYCLHDGV CMYIEALDKY ACNCVVGYIG ERCQYRDLKW WELR

UniProt No.: P01133
NCBI Accession No.: NP_001954.2

Molecular Weight: 6.3 kDa (54aa) confirmed by MALDI-TOF
Concentration: 1mg/ml (determined by Bradford assay)
Formulation: Liquid in. Phosphate-Buffered Saline (pH 7.4)
Purity: > 95% by SDS-PAGE
Bioactivity: Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 range ≤ 0.1 ng/ml.
Endotoxin: < 1 EU per 1ug of protein (determined by LAL method)
Tag: Non-Tagged
Storage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.