EGF monoclonal, anti-mouse
€0.00
In stock
SKU
AC-ABG10069
Source:
Produced in Sprague Dawley rats using highly pure (>98%) recombinant murine EGF as the immunizing antigen. This IgG2a kappa antibody was purified from cell culture by Protein G affinity chromatography.
Western Blot:
To detect mEGF by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mEGF is 0.25-0.50 ng/lane under non-reducing conditions and 10.0-15.0 ng/lane under reducing conditions.
Sandwich ELISA:
In a sandwich ELISA (assuming 100 μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 100 pg/ml of recombinant murine EGF when used with BioGems biotinylated antigen affinity purified anti-murine EGF (61-063BT) as the detection antibody at a concentration of approximately 1.0-2.0 μg/ml.
Formulation: A sterile filtered antibody solution was lyophilized from PBS.
Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Produced in Sprague Dawley rats using highly pure (>98%) recombinant murine EGF as the immunizing antigen. This IgG2a kappa antibody was purified from cell culture by Protein G affinity chromatography.
Western Blot:
To detect mEGF by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mEGF is 0.25-0.50 ng/lane under non-reducing conditions and 10.0-15.0 ng/lane under reducing conditions.
Sandwich ELISA:
In a sandwich ELISA (assuming 100 μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 100 pg/ml of recombinant murine EGF when used with BioGems biotinylated antigen affinity purified anti-murine EGF (61-063BT) as the detection antibody at a concentration of approximately 1.0-2.0 μg/ml.
Formulation: A sterile filtered antibody solution was lyophilized from PBS.
Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
| Is Featured? | No |
|---|
Write Your Own Review