EGF Receptor polyclonal, anti-human

EGF Receptor polyclonal, anti-human

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In stock
SKU
AC-ABG10070
Catalog Number: AC-ABG10070
Size(s): 50 µg, 100 µg
Host: Rabbit
Applications: WB, E
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Source:
Produced from sera of rabbits immunized with highly pure recombinant Human EGF Receptor. Anti-Human EGF Receptor specific antibody was purified by affinity chromatography employing an immobilized Human EGF Receptor matrix.

Western Blot:
To detect Human EGF Receptor by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human EGF Receptor is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Sandwich ELISA:
To detect Human EGF Receptor by sandwich ELISA (using 100 μl/welll antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems Biotinylated Anti-Human EGF Receptor (60-116BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human EGF Receptor.

Formulation: A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.

Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
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