EGFR (phospho-Y992) polyclonal, anti-human, mouse, rat
€328.00
In stock
SKU
BS4310
Background:
Epidermal growth factor mediates its effects on cell growth through its interaction with a cell surface glycoprotein designated the EGF receptor. Binding of EGF or TGFα to the EGF receptor activates tyrosine-specific protein kinase activity intrinsic to the EGF receptor. The carboxy-terminal tyrosine residues on EGFR, Tyr 1068 and Tyr 1173, are the major sites of autophosphorylation, which occurs as a result of EGF binding. Phosphorylation of Tyr 992, Tyr 1068 and Tyr 1086 is required for conformational change in the C-terminal tail of the EGF receptorgulates Actin filament accumulation at the plasma membrane and Cdc42 stimulates formation of filopodia.
Alternative Name:
Epidermal growth factor receptor, EC=2.7.10.1, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, ERBB, ERBB1, HER1, EGFR
Application Dilution: WB: 1:500~1:1000, IHC: 1:50~1:200, IF: 1:50~1:200
Specificity: p-EGFR (Y992) polyclonal antibody detects endogenous levels of EGFR protein only when phosphorylated at Tyr992 (removal of the signal peptide).
Immunogen:
Synthetic phosphopeptide derived from human EGFR around the phosphorylation site of Tyrosine 992.
MW: ~ 175 kDa
Swis Prot.: P00533
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1mg/ml in PBS with 0.1% Sodium Azide, 50% Glycerol.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
Epidermal growth factor mediates its effects on cell growth through its interaction with a cell surface glycoprotein designated the EGF receptor. Binding of EGF or TGFα to the EGF receptor activates tyrosine-specific protein kinase activity intrinsic to the EGF receptor. The carboxy-terminal tyrosine residues on EGFR, Tyr 1068 and Tyr 1173, are the major sites of autophosphorylation, which occurs as a result of EGF binding. Phosphorylation of Tyr 992, Tyr 1068 and Tyr 1086 is required for conformational change in the C-terminal tail of the EGF receptorgulates Actin filament accumulation at the plasma membrane and Cdc42 stimulates formation of filopodia.
Alternative Name:
Epidermal growth factor receptor, EC=2.7.10.1, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, ERBB, ERBB1, HER1, EGFR
Application Dilution: WB: 1:500~1:1000, IHC: 1:50~1:200, IF: 1:50~1:200
Specificity: p-EGFR (Y992) polyclonal antibody detects endogenous levels of EGFR protein only when phosphorylated at Tyr992 (removal of the signal peptide).
Immunogen:
Synthetic phosphopeptide derived from human EGFR around the phosphorylation site of Tyrosine 992.
MW: ~ 175 kDa
Swis Prot.: P00533
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1mg/ml in PBS with 0.1% Sodium Azide, 50% Glycerol.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
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