eIF-2a (Phospho-S51) polyclonal, anti-human, mouse, rat

Background:
Phosphorylation of the eukaryotic initiation factor 2 (eIF2) α subunit is a well-documented mechanism to downregulate protein synthesis under a variety of stress conditions. Eukaryotic initiation factor 2 binds GTP and Met-tRNAi and transfers Met-tRNA to the 40S subunit to form the 43S preinitiation complex. eIF2 promotes a new round of translation initiation by exchanging GDP for GTP, a reaction catalyzed by eIF2B. Kinases that are activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2), or heme deficiency (HRI) can phosphorylate the α subunit of eIF2. This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51.

Alternative Name:
EIF2A, CDA02, MSTP004, MSTP089, EIF 2 alpha, EIF 2, EIF 2A, EIF 2alpha, eIF-2-alpha, eIF-2A, EIF-2alpha, EIF2 alpha, EIF2, EIF2A, EIF2S1, Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa, Eukaryotic translation initiation factor 2 subunit 1 alpha, Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit alpha, IF2A,

Application Dilution:
WB:1:1,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Specificity: eIF-2a (Phospho-S51) polyclonal antibody detects endogenous levels of eIF-2a protein only when phosphorylated at S51.

Immunogen:
Synthetic phospho-peptide corresponding to residues surrounding Ser51 of human eIF-2a.

MW: 36 kDa

Swiss-Prot: Q9BY44(Human) Q8BJW6(Mouse) EntrezGene:502531(Rat)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.