EIF3A Polyclonal Antibody

Background:
RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis . The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632, PubMed:11169732). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.

Synonyms:
EIF3A, EIF3, EIF3S10, P167, TIF32, eIF3-p170, eIF3-theta, p180, p185

Immunogen: Recombinant fusion protein of human EIF3A (NP_003741.1).

Swissprot: Q14152
Gene ID: 8661

Calculated MW: 162 kDa/166 kDa
Observed MW: 180 kDa

Purification Method: Affinity purification

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3

Concentration: 1 mg/mL
Dilution: "WB 1:500-1:1000 IHC 1:50-1:100 IF 1:50-1:100"

Storage:
Store at -20°C. Avoid freeze / thaw cycles.