eIF4E (Phospho-S209) polyclonal, anti-human, mouse, rat

Background:
The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex eIF4F exists in vitro as a trimeric complex of eIF4E, eIF4A and eIF4G. Together, the complex allows ribosome binding to mRNA by inducing the unwinding of mRNA secondary structures. eIF4E binds to the mRNA "cap" during an early step in the initiation of protein synthesis. eIF4A acts as an ATP-dependent RNA helicase. eIF4G acts as a bridge between eIF4E, eIF4A and the eIF3 complex.

Alternative Name:
eIF-4E, eIF4E, EIF4E, AUTS19, CBP, eIF 4E, eIF 4F 25 kDa subunit, EIF 4F, eIF-4E, eIF-4F 25 kDa subunit, eIF4E, EIF4E1, EIF4EL1, EIF4F, Eukaryotic translation initiation factor 4 E, Eukaryotic translation initiation factor 4E, Eukaryotic translation initiation factor 4E like 1, IF4E, Messanger RNA Cap Binding Protein eIF 4E, MGC111573, mRNA cap binding protein, mRNA cap-binding protein,

Application Dilution:
WB:1:1,000-1:5,000
ICC:1:50-1:200
IHC:1:50-1:200

Specificity: eIF4E (Phospho-S209) polyclonal antibody detects endogenous levels of eIF4E protein only when phosphorylated at S209.

Immunogen:
Synthetic phospho-peptide corresponding to residues surrounding Ser209 of human eIF4E.

MW: 25 kDa

Swiss-Prot: P06730(Human) P63073(Mouse) P63074(Rat)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.