eIF4E polyclonal, anti-human, mouse, rat
€388.00
In stock
SKU
BS6591
Background:
The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex eIF4F exists in vitro as a trimeric complex of eIF4G, eIF4E, and eIF4A. Together, the complex allows ribosome binding to mRNA by inducing the unwinding of mRNA secondary structures. eIF4E binds to the mRNA “cap” during an early step in the initiation of protein synthesis . eIF4A acts as an ATP-dependent RNA helicase. eIF4G acts as a bridge between eIF4E, eIF4A, and the eIF3 complex.
Alternative Name:
eIF4E ( phospho S209 ), eIF-4E, eIF4E, mRNA cap-binding protein, eIF-4F 25 kDa subunit, EIF4E, 1977, EIF4E, 133440, P06730, CBP, EIF4EL1, EIF4F, MGC111573,
Application Dilution: WB: 1:500 - 1:2000, IHC/IF: 1:50 - 1:100, IP: 1:50 - 1:100
Specificity: EIF4E polyclonal antibody detects endogenous levels of EIF4E protein.
Immunogen:
Recombinant full length Human eIF4E.
MW: ~ 25,30 kDa
Swis Prot.: P06730
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1mg/ml in PBS with 0.1% Sodium Azide, 50% Glycerol.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex eIF4F exists in vitro as a trimeric complex of eIF4G, eIF4E, and eIF4A. Together, the complex allows ribosome binding to mRNA by inducing the unwinding of mRNA secondary structures. eIF4E binds to the mRNA “cap” during an early step in the initiation of protein synthesis . eIF4A acts as an ATP-dependent RNA helicase. eIF4G acts as a bridge between eIF4E, eIF4A, and the eIF3 complex.
Alternative Name:
eIF4E ( phospho S209 ), eIF-4E, eIF4E, mRNA cap-binding protein, eIF-4F 25 kDa subunit, EIF4E, 1977, EIF4E, 133440, P06730, CBP, EIF4EL1, EIF4F, MGC111573,
Application Dilution: WB: 1:500 - 1:2000, IHC/IF: 1:50 - 1:100, IP: 1:50 - 1:100
Specificity: EIF4E polyclonal antibody detects endogenous levels of EIF4E protein.
Immunogen:
Recombinant full length Human eIF4E.
MW: ~ 25,30 kDa
Swis Prot.: P06730
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1mg/ml in PBS with 0.1% Sodium Azide, 50% Glycerol.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
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