EIF6 polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
EIF6 (Eukaryotic Translation Initiation Factor 6), also called EIF3A or ITGB4BP, is a human gene. By fluorescence in situ hybridization, Sanvito et al. (1998) mapped the ITGB4BP gene to 20q11.2. Ceci et al. (2003) demonstrated that the ribosomal 60S subunit is activated by release of EIF6. In the cytoplasm, EIF6 is bound to free 60S but not to 80S subunits. Furthermore, EIF6 interacts in the cytoplasm with RACK1, a receptor for activated protein kinase C. Gandin et al. (2008) demonstrated that mammalian eIF6 is required for efficient initiation of translation in vivo. Eif6-null mouse embryos were lethal at preimplantation. Heterozygous mice had 50% reduction of eIF6 levels in all tissues, and showed reduced mass of hepatic and adipose tissues due to a lower number of cells and to impaired G1/S cell cycle progression.

Description:
Rabbit IgG polyclonal antibody for Eukaryotic translation initiation factor 6 (EIF6) detection. Tested with WB, IHC-P in Human, Mouse, Rat.

Synonyms:
b(2)gcn antibody, B(2)GCN homolog antibody, B4 integrin interactor antibody, Binding protein of beta-4 integrin antibody, CAB antibody, eIF-6 antibody, EIF3A antibody, EIF6 antibody, Eukaryotic translation initiation factor 3A antibody, Eukaryotic translation initiation factor 6 antibody, IF6_HUMAN antibody, ITGB4BP antibody, OK/SW-cl.27 antibody, p27 beta 4 integrin binding protein antibody, p27(BBP) antibody, p27BBP antibody, RP4-614O4.1 antibody.

Isotype: Rabbit polyclonal IgG

Immunogen:
E.coli-derived human EIF6 recombinant protein (Position: N66-T210). Human EIF6 shares 99.3% amino acid (aa) sequence identity with both mouse and rat EIF6.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, mouse, rat

Applications: WB, IHC-P

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.