EKLF/CKLF/UKLF rabbit polyclonal, anti-human, mouse, rat

Backgroud: This gene encodes a hematopoietic-specific transcription factor that induces high-level expression of adult beta-globin and other erythroid genes. The zinc-finger protein binds to the DNA sequence CCACACCCT found in the beta hemoglobin promoter. Heterozygous loss-of-function mutations in this gene result in the dominant In(Lu) blood phenotype. [provided by RefSeq, Oct 2009],function:Transcription regulator of erythrocyte development. Binds to the CACCC box in the beta-globin gene promoter and activates transcription. When sumoylation, acts as a Probably serves as a general switch factor for erythroid development. When sumoylated, acts as a transcriptional repressor, by promoting interaction with CDH2/MI2beta and also represses megakaryocytic differentiation.,PTM:Acetylated; can be acetylated on both Lys-274 and Lys-288. Acetylation on Lys-274 (by CBP) appears to be the major site affecting EKLF transactivation activity.,PTM:Phosphorylated primarily on serine residues in the transactivation domain. Phosphorylation on Thr-23 is critical for the transactivation activity.,PTM:Sumoylated; sumoylation, promoted by PIAS1, leads to repression of megakaryocyte differentiation. Also promotes the interaction with the CDH4 subunit of the NuRD repression complex.,similarity:Belongs to the krueppel C2H2-type zinc-finger protein family.,similarity:Contains 3 C2H2-type zinc fingers.,subcellular location:Colocalizes with SUMO1 in nuclear speckles.,subunit:Interacts with CBP and EP300; the interactions enhance the transactivation activity. Interacts with PCAF; the interaction does not acetylate EKLF and inhibits its transactivation activity.,tissue specificity:Expression restricted to adult bone marrow and fetal liver. Not expressed in myeloid nor lymphoid cell lines.,

Synonyms: KLF1, EKLF, Krueppel-like factor 1, Erythroid krueppel-like transcription factor, EKLF, KLF5, BTEB2, CKLF, IKLF, Krueppel-like factor 5, Basic transcription element-binding protein 2, BTE-binding protein 2, Colon krueppel-like factor, GC-bo

Gene Name: KLF1/KLF5/KLF7

Gene ID: 10661/688/8609

SwissProt ID: Q13351/Q13887/O75840

Purification: Affinity purification

Storage: Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.