ENDOG polyclonal, anti-human, mouse, rat
€305.00
In stock
SKU
BS60425
Background:
Endonuclease G (ENDOG), a nuclear encoded protein, localizes to the mitochondria (1). This sugar-nonspecific nuclease, responsible for major mitochondrial nuclease activity, preferentially cleaves single-stranded DNA(ssDNA). Synthesized as a propeptide with an amino-terminal presequence that targets the nuclease to mitochondria, ENDOG translocates to neuclei on apoptotic stimulation and act as a nuclease without sequence specificity. Both exonucleases and DNase I stimulate the ability of ENDOG to generate double-stranded DNA cleavage products at physiological ionic strengths, suggesting that these activities work in concert with ENDOG in apoptotic cells to ensure efficient DNA breakdown.
Alternative Name:
Endonuclease G, mitochondrial, Endo G
Application Dilution: WB: 1:500~1:1000
Specificity: ENDOG polyclonal antibody detects endogenous levels of ENDOG protein.
Immunogen:
A synthetic peptide corresponding to residues in Human ENDOG.
MW: ~ 35 kDa
Swis Prot.: Q14249
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1mg/ml in PBS with 0.1% Sodium Azide, 50% Glycerol.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
Endonuclease G (ENDOG), a nuclear encoded protein, localizes to the mitochondria (1). This sugar-nonspecific nuclease, responsible for major mitochondrial nuclease activity, preferentially cleaves single-stranded DNA(ssDNA). Synthesized as a propeptide with an amino-terminal presequence that targets the nuclease to mitochondria, ENDOG translocates to neuclei on apoptotic stimulation and act as a nuclease without sequence specificity. Both exonucleases and DNase I stimulate the ability of ENDOG to generate double-stranded DNA cleavage products at physiological ionic strengths, suggesting that these activities work in concert with ENDOG in apoptotic cells to ensure efficient DNA breakdown.
Alternative Name:
Endonuclease G, mitochondrial, Endo G
Application Dilution: WB: 1:500~1:1000
Specificity: ENDOG polyclonal antibody detects endogenous levels of ENDOG protein.
Immunogen:
A synthetic peptide corresponding to residues in Human ENDOG.
MW: ~ 35 kDa
Swis Prot.: Q14249
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1mg/ml in PBS with 0.1% Sodium Azide, 50% Glycerol.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
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