eNOS (Phospho-Thr494) polyclonal, anti-human, mouse, rat

Specificity:
eNOS (Phospho-Thr494) Antibody detects endogenous levels of NOS3 only when phosphorylated at Thr494.

Synonyms: cNOS, Constitutive NOS, EC-NOS, ECNOS, Endothelial NOS, Nitric-oxide synthase, endothelial, NOS, type III, NOS3, NOSIII

Immunogen:
The antiserum was produced against synthesized phospho-peptide derived from human eNOS around the phosphorylation site of Thr494.

Immunogen Sequence:
Phospho

Modification Sites: H:T495, M:T494, R:T494

MW (kDa): 133 kDa

NCBI Gene Symbol: NOS3

Database Links:
Gene ID: 4846
Swiss Prot No.: P29474
OMIM No.: 163729
Unigene No.: Hs.647092

Application Dilutions: WB: 1:500~1:1000, ELISA: 1:10000

Purification:
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Physical Format:
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Concentration: 1 mg/ml

Storage: Stable at -20°C for at least 1 year.