eNOS polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
NOS3(Nitric Oxide Synthase 3), also called ENOS, a nitric oxide synthase that generates NO in blood vessels and is involved with regulating vascular tone by inhibiting smooth muscle contraction andplatelet aggregation. The NOS3 gene is mapped on 7q36.1. Variations in this gene are associated with susceptibility to coronary spasm. Fulton et al.(1999) concluded the eNOS is an AKT substrate linking signal transduction by AKT to the release of the gaseous second messenger nitric oxide. AKT mediates the activation of eNOS, leading to increased nitric oxide production. Inhibition of the PI3K AKT pathway or mutation of the AKT site on eNOS protein at serine-1177 attenuated the serine phosphorylation and prevented the activation of eNOS. RT-PCR analysis showed that expression of NOS3 in human umbilical vein endothelial cells(HUVECs) and human aortic vascular smooth muscle cells(HAOVSMCs) was inversely proportional to that of NOS3AS.

Description:
Rabbit IgG polyclonal antibody for Nitric oxide synthase, endothelial (NOS3) detection. Tested with WB, IHC-P in Human, Mouse, Rat.

Synonyms:
cNOS antibody, Constitutive NOS antibody, EC NOS antibody, EC-NOS antibody, ecNOS antibody, Endothelial nitric oxidase synthase antibody, Endothelial nitric oxide synthase antibody, Endothelial nitric oxide synthase 3 antibody, Endothelial NOS antibody, eNOS antibody, Nitric oxide synthase 3(endothelial cell) antibody, Nitric oxide synthase 3 antibody, Nitric oxide synthase 3 endothelial cell antibody, Nitric oxide synthase endothelial antibody, Nitric oxide synthase, endothelial antibody, NOS 3 antibody, NOS III antibody, NOS type III antibody, NOS3 antibody, NOS3_HUMAN antibody, NOSIII antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the N-terminus of human eNOS(144-165 QAHEQRLQEVEAEVAATGTYQL), identical to the related mouse sequence, and different from the related rat sequence by one sequence.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human.
Predicted to work with: mouse, rat.

Applications: WB, IHC-P

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.