Eotaxin monoclonal, anti-human

Source:
Produced in BALB/c mice using highly pure (>98%) recombinant human Eotaxin as the immunizing antigen. This IgG1k antibody was purified from cell culture by Protein A affinity chromatography.

Western Blot:
To detect hEotaxin by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin is 0.5-1.0 ng/lane, under reducing or non-reducing conditions.

Sandwich ELISA:
In a sandwich ELISA (assuming 100 μl/well), a concentration of 1.0-2.0 μg/ml of this antibody will detect at least 800 pg/ml of recombinant human Eotaxin when used with BioGems biotinylated antigen affinity purified anti-human Eotaxin (60-122GBT) as the detection antibody at a concentration of approximately 0.5-1.0 μg/ml.

Formulation: A sterile filtered antibody solution was lyophilized from PBS.

Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.