EpCAM (Extracellular region) (clone M039), anti-human

Background:
Epithelial Cell Adhesion Molecule (EpCAM) is a signal type I transmembrane glycoprotein that has an extracellular domain with one thyroglobulin type-1 domain and a short cytoplasmic domain. EpCAM is found on the surface of adenocarcinoma, but not on mesodermal or neural cell membranes. The EpCAM molecule has been shown to function as a homophilic Ca2+ independent adhesion molecule. It may act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium. Defects in EpCAM cause hereditary non-polyposis colorectal cancer type 8 (HNPCC8) and diarrhea type 5 (DIAR5). EpCAM plays a role in embryonic stem cell proliferation and differentiation; it up-regulates the expression of FABP5, MYC, and Cyclin A & Cyclin E. It is highly and selectively expressed by undifferentiated embryonic stem cells and in many types of epithelial carcinomas.

Sequence: Clone (M039) was generated from the extracellular region of human EpCAM.
*For more information, see UniProt Accession P16422

Specificity: Clone M039 mouse monoclonal antibody detects a 35-45 kDa* protein on SDS-PAGE "Native" immunoblots of human A431, H1915, and MCF7 carcinomas. This antibody weakly detects denatured EpCAM. The antibody works for native western blot, immunoprecipitation, ELISA capture, and immunocytochemistry.

Application dilution:
ELISA (Cap) 1:100
ICC 1:100
IP 1:100
WB 1:1000
End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.

Buffer/Storage:
Mouse monoclonal antibody purified with protein G chromatography is supplied in 100 µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.