Ephexin-1 (unphosphorylated Tyr-87) Blocking Peptide
€155.00
In stock
SKU
ECM-EX2855
Background:
The Eph family of receptor tyrosine kinases, and their ephrin ligands, are important for cell positioning and morphogenesis during development. EphA4 receptor can inhibit axon outgrowth and has roles in regulating axon projections during neural development. Eph signaling pathways require both receptor kinase activity and activation of Rho-GTPase guanine nucleotide-exchange factors (GEFs). Ephexin-1 is a Dbl family GEF that may be important for regulating Rho GTPase activity downstream of EphA4/FGFR complexes. EphA4 activation leads to Src kinase phosphorylation of Tyr-87 in ephexin-1, which enhances activation of RhoA, but not Rac1 or Cdc42. In addition, ephexin-1 also binds FGFRs, and is phosphorylated at Tyr-87, as well as other tyrosine sites.
Sequence: Unphosphorylated ephexin-1 (Tyr-87) synthetic peptide corresponding to amino acids surrounding tyrosine 87 in mouse ephexin-1. This sequence has significant homology to the conserved region in human and rat ephexin-1.
Specificity: This peptide is an unphosphorylated control peptide for Ephexin-1 (Tyr-87) phospho-specific antibody (EP2841). The peptide can be used as a negative control for phosphorylated Ephexin-1 (Tyr-87) peptide (EX2845) in ELISA, Western blot and immunocytochemistry.
Buffer/Storage:
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
The Eph family of receptor tyrosine kinases, and their ephrin ligands, are important for cell positioning and morphogenesis during development. EphA4 receptor can inhibit axon outgrowth and has roles in regulating axon projections during neural development. Eph signaling pathways require both receptor kinase activity and activation of Rho-GTPase guanine nucleotide-exchange factors (GEFs). Ephexin-1 is a Dbl family GEF that may be important for regulating Rho GTPase activity downstream of EphA4/FGFR complexes. EphA4 activation leads to Src kinase phosphorylation of Tyr-87 in ephexin-1, which enhances activation of RhoA, but not Rac1 or Cdc42. In addition, ephexin-1 also binds FGFRs, and is phosphorylated at Tyr-87, as well as other tyrosine sites.
Sequence: Unphosphorylated ephexin-1 (Tyr-87) synthetic peptide corresponding to amino acids surrounding tyrosine 87 in mouse ephexin-1. This sequence has significant homology to the conserved region in human and rat ephexin-1.
Specificity: This peptide is an unphosphorylated control peptide for Ephexin-1 (Tyr-87) phospho-specific antibody (EP2841). The peptide can be used as a negative control for phosphorylated Ephexin-1 (Tyr-87) peptide (EX2845) in ELISA, Western blot and immunocytochemistry.
Buffer/Storage:
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
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