ERBB2 Polyclonal Antibody

Background:
Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.

Research Area: Cancer, Immunology, Signal Transduction, Tags and Cell Markers

Synonyms:
Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog, C erb B2/neu protein, CD340, CD340 antigen, Cerb B2/neu protein, CerbB2, Erb b2 receptor tyrosine kinase 2, ERBB2, ERBB2, HER 2, HER 2/NEU, HER2, Herstatin, Human epidermal growth factor receptor 2, Metastatic lymph node gene 19 protein, MLN 19, MLN19, NEU, NEU proto oncogene, Neuro/glioblastoma derived oncogene homolog, Neuroblastoma/glioblastoma derived oncogene homolog, NGL, p185erbB2, Proto-oncogene c-ErbB-2, Proto-oncogene Neu, Receptor tyrosine-protein kinase erbB-2, TKR1, Tyrosine kinase type cell surface receptor HER2, Tyrosine kinase-type cell surface receptor HER2, V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog), V erb b2 avian erythroblastic leukemia viral oncogene homolog 2, V erb b2 avian erythroblastic leukemia viral oncoprotein 2, V erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian), V erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog, Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)

Immunogen: Synthesized peptide derived from the Internal region of human Neu

Swissprot: P04626

Calculated MW: 138 kDa
Observed MW: 138 kDa

Purification Method: Affinity purification

Buffer: PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol pH 7.4.

Concentration: 1 mg/mL
Dilution: WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000, ELISA 1:20000

Storage:
Store at -20°C. Avoid freeze / thaw cycles.