ERK1/2 (Phospho-T202) polyclonal, anti-human

Background:
Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs, such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines, and research investigators consider it an important target in the diagnosis and treatment of cancer. Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family, as well as Mos and Tpl2/COT. MEK1 and MEK2 are the primary MAPKKs in this pathway. MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK and the transcription factor Elk-1. p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs, along with MEK inhibitors, such as U0126 and PD98059.

Alternative Name:
MAP kinase 3, MAPK 3, ERK-1, p44-MAPK, MAPK3, ERK1, PRKM3, MAP kinase 1, MAPK 1, ERK-2, p42-MAPK, MAP kinase 2, MAPK 2, MAPK1, ERK2, PRKM1, PRKM2,

Application Dilution:
WB:1:1,000
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:50-1:100

Specificity: ERK1/2 (Phospho-T202) polyclonal antibody detects endogenous levels of ERK1/2 protein only when phosphorylated at T202.

Immunogen:
Synthetic phospho-peptide corresponding to residues surrounding Thr185 of human Erk2.

MW: 42/44 kDa

Swiss-Prot: P27361(Human) P28482(Human)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.