Ethanol Colorimetric Assay Kit

Detection Principle:
Ethanol dehydrogenase can catalyze oxidative dehydrogenation of ethanol to acetaldehyde, and NAD+ is reduced to produce NADH. NADH, under the action of 1-mPMS, transfer electrons to WST-8 to produce the yellow product, which has a characteristic absorption peak at 450 nm. Therefore, ethanol content can be quantified by measure the OD value at 450 nm.
Instrument: Microplate reader
Assay Time: 30 min

Tested Sample Types: Serum, plasma, wine

Research Area's: Others

Shelf Life: 3 Months

Storage:
-20℃