EZH2 Polyclonal Antibody

Background:
EZH2 (enhancer of zeste homologue 2, also known as KMT6) is a member of Polycomb group (PcG) family and encodes a histone methyl transferase that has an essential role in promoting histone H3 lysine 27 trimethylation (H3K27me3) and epigenetic gene silencing. EZH2 is important for cell proliferation and inhibition of cell differentiation, and is implicated in cancer progression. Overexpression of EZH2 is a marker of advanced and metastatic disease in many solid tumors, including prostate and breast cancer. This antibody detected EZH2 protein as a single band with a molecular weight (MW) of 91-100 kDa in multiple cell lines. The phosphorylation may result in the higher molecular weight (calculated MW as 80-86 kDa). (20935635, 21367748)

Research Area: Cancer, Epigenetics and Nuclear Signaling

Synonyms:
Enhancer of zeste 2, enhancer of zeste 2 polycomb repressive complex 2 subunit, Enhancer of zeste homolog 2 (Drosophila), Enhancer of zeste homolog 2, Enhancer of zeste, Drosophila, homolog 2, ENX 1, Enx 1h, ENX-1, ENX1, Enx1h, EZH 2, EZH1, EZH2, EZH2, EZH2b, Histone-lysine N-methyltransferase EZH2, KMT 6, KMT6, KMT6A, Lysine N-methyltransferase 6, MGC9169, WVS, WVS2

Immunogen: KLH conjugated Synthetic peptide corresponding to Mouse EZH2

Swissprot: Q15910,Q61188

Calculated MW: 80-100 kDa
Observed MW: 80 kDa

Purification Method: Affinity purification

Buffer: PBS with 0.02% sodium azide, 1% BSA and 50% glycerol, pH7.4

Concentration: 0.54 mg/mL
Dilution: WB 1:500-1:2000

Storage:
Store at -20°C. Avoid freeze / thaw cycles.