Factor IX / F9 Polyclonal Antibody

Background:
This gene encodes vitamin K-dependent coagulation factor IX that circulates in the blood as an inactive zymogen. This factor is converted to an active form by factor XIa, which excises the activation peptide and thus generates a heavy chain and a light chain held together by one or more disulfide bonds. The role of this activated factor IX in the blood coagulation cascade is to activate factor X to its active form through interactions with Ca+2 ions, membrane phospholipids, and factor VIII. Alterations of this gene, including point mutations, insertions and deletions, cause factor IX deficiency, which is a recessive X-linked disorder, also called hemophilia B or Christmas disease. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing.

Research Area: Cardiovascular, Immunology

Synonyms:
F9, F9 p22, FIX, HEMB, P19, PTC, THPH8, F9p22

Immunogen: Recombinant fusion protein of human Factor IX / F9 (NP_000124.1).

Swissprot: P00740
Gene ID: 2158

Calculated MW: 47 kDa/51 kDa
Observed MW: 50 kDa

Purification Method: Affinity purification

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3

Concentration: 1 mg/mL
Dilution: "WB 1:500-1:2000 IF 1:50-1:200"

Storage:
Store at -20°C. Avoid freeze / thaw cycles.