Factor X polyclonal, anti-human

Quantity: 100 µl

Background:
The defined antibody reactivity is restricted to Factor X as tested at the level of sensitivity of immuno-precipitation techniques. A single precipitin line is obtained in bidimensional electrophoresis, immunoelectrophoresis and double radial immunodiffusion (Ouchterlony) which shows a reaction of full identity with the purified immunogen. In precipitating techniques as electroimmunodiffusion, immunoelectrophoresis and single and double radial immnodiffusion (Mancini, Ouchterlony). To prepare an adsorbent for immunoaffinity purification of FIX. If used in more sensitive test procedures or as catching or detection antibody in solid phase immunoassays specificity controls should always be include. Plasma samples and all assay components must contain EDTA to stabilize the proteins.

Isotype: Rabbit Polyclonal

Source: Plasma Factor X is a relatively stable glycoprotein with alpha-1 mobility in electrophoresis. It ha s a molecular weight of 56,000, consists of a heavy chain and a light chain, and is essential for normal clotting and haemoastasis. FX can be activated by both the intrinsic and the extrinsic pathways. It is converted to Fxa by FIXa and FVIII in the presence of calcium ions and phospholipid (intrinsic pathway), by tissue factor and FVIIIa (extrinsic pathway), or by direct platelet action. After coagulation, FX antigen is present in serum. Fxa is activated by combining irreversibly with antithrombin III, with heparin acting as cofactor. Norma adult plasma contains an avaerage of 0.5 to 1 mgFX per 100 ml. Increased adult levels are seen during normal pregnancy and with the use of oral contraceptives. Deficiency of FX may be congenital or acquired and may result in a haemorrhagic disorder. Several types of congenital deficiencies are known: reduced FX activity and low antigen levels (Stuart), or with synthesis of abnormal molecules (Prower); abnormal molecular variants may have a selective deficiency in response to one or several activators. Acquired FX deficiency results mostly from vitamin K deficiency. During oral anticoagulant therapy FX deficiency usually develops as part of a combined deficiency with FII, FVI and FIX and with the appearance of inactive factors which lack calcium binding sites. FX deficiency may also occur in primary ubt probably not in secondary amyloidosis. For immunization FX is obtained in highly purified form from pooled fresh plasma. Freund's complete adjuvant is used in the first step of the immunization procedure.

Form: Delipidated, heat inactivated, in PBS and 0.1% sodium azide.

Reactivity: human

Cross Reactivity:
The antiserum does not cross-react with any other Human plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.

Applications: Immunoprecipitation

Storage: Store at 4C, or in small aliquots at -20C.