FANCD2 polyclonal, anti-human, mouse, rat

Background:
Fanconi anemia (FA) is an autosomal recessive disorder character-ized by bone marrow failure, birth defects and chromosomal instability. At the cellular level, FA is characterized by spontaneous chromosomal breakage and a unique hypersensitivity to DNA cross-linking agents. At least 8 complementation groups (A-G) have been identified and 6 FA genes (for subtypes A, C, D2, E, F and G) have been cloned. The FA proteins lack sequence homologies or motifs that could point to a molecular function. Phosphorylation of FANC (Fanconi anemia complementation group) proteins are thought to be important for the function of the FA pathway. Several FA proteins, including FANCA, FANCC, FANCF, and FANCG, interact in a nuclear complex, and this complex is required for the activation (monoubiquitination) of the downstream FANCD2 protein. When monoubiquitinated, the FANCD2 protein co-localizes with the breast cancer susceptibility protein BRCA1 in DNA damage induced foci. In male meiosis, FANCD2 also co-localizes with BRCA1 at synaptonemal complexes. The human FANCD2 gene maps to chromosome 3p25.3, contains 44 exons and encodes a 1,451-amino acid nuclear protein that exists as 2 protein isoforms.

Alternative Name:
FANCD2, Protein FACD2, DKFZp762A223, FA 4, FA D2, FA4, FAC D2, FACD 2, FACD, FACD2, FACD2, FAD, FAD2, FANC D2, FANCD 2, FANCD, FANCD2, FANCONI ANEMIA COMPLEMENTATION GROUP D, Fanconi anemia complementation group D2, Fanconi anemia group D2 protein, FANCONI PANCYTOPENIA TYPE 4, FLJ23826, OTTHUMP00000158853, OTTHUMP00000207925, Protein FACD2, Type 4 Fanconi pancytopenia,

Application Dilution:
WB:1:500-1:1,000
ICC:1:100-1:500
IHC:1:50-1:200

Specificity: FANCD2 polyclonal antibody detects endogenous levels of FANCD2 protein.

Immunogen:
recombinant protein

MW: 166 kDa

Swiss-Prot: Q9BXW9(Human) Q80V62(Mouse) Q6IV68(Rat)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.