FBXW7 polyclonal, anti-human, mouse, rat

Background:
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds phosphorylated sites/phosphodegrons within target proteins and thereafter bring them to the SCF complex for ubiquitination. Identified substrates include cyclin-E (CCNE1 or CCNE2), JUN, MYC, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. Acts as a negative regulator of JNK signaling by binding to phosphorylated JUN and promoting its ubiquitination and subsequent degradation

Alternative Name:
hAgo, FBXW7, FBW7, AGO, Archipelago, Archipelago homolog, Archipelago, Drosophila, homolog of, CDC4, CDC4, S.cerevisiae, homolog of, DKFZp686F23254, F box and WD 40 domain protein 7 (archipelago homolog, Drosophila), F box and WD 40 domain protein 7, F box and WD repeat domain containing 7, F box protein FBW7, F box protein FBX30, F box protein SEL 10, F box protein SEL10, F-box and WD repeat domain containing 7, E3 ubiquitin protein ligase, F-box and WD-40 domain-containing protein 7, F-box protein FBX30, F-box/WD repeat-containing protein 7, FBW6, FBW7, FBX30, FBXO30, FBXW6, FBXW7, FBXW7, FLJ16457, hAgo, hCdc4, Homolog of C elegans sel 10, Homolog of C.elegans sel10, SEL 10, SEL-10, SEL10,

Application Dilution:
WB:1:500-1:1,000
ICC:1:50-1:2,00
FC:1:50-1:100
IHC:1:50-1:200

Specificity: FBXW7 polyclonal antibody detects endogenous levels of FBXW7 protein.

Immunogen:
Recombinant protein

MW: 70 kDa

Swiss-Prot: Q969H0(Human) Q8VBV4(Mouse) D3Z902(Rat)

Purification & Purity:
Protein affinity purified.

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.