Fc gamma RIIA/CD32a (R167) human recombinant protein

Background:
Fc gamma RIIA/CD32a, also known as Low affinity immunoglobulin gamma Fc region receptor II-a, is a member of the Ig superfamily that function in the activation or inhibition of immune responses. CD32 is expressed on many immune cell types - notably, CD32a is found on platelets, neutrophils, macrophages, and dendritic cells. CD32 has a low-affinity for the Fc region of IgG antibodies in monomeric form, but high affinity for IgG immune complexes. Besides IgG, it also binds C-reactive protein (CRP). Two allelic variants of CD32a, that differ in their ability to ligate human IgG2 or CRP exist. CD32 has two major functions: cellular response regulation, and the uptake of immune complexes. Cellular responses regulated by CD32 include phagocytosis, cytokine stimulation, and endocytic transport. Dysregulated CD32 is associated with different forms of autoimmunity, including systemic lupus erythematosus. Recombinant human Fc gamma RIIA/CD32a, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Alternative Names:
Low affinity immunoglobulin gamma Fc region receptor II-a, IgG Fc receptor II-a, IgG Fc fragment receptor, CDw32, Fc-gamma RII-a, Fcgamma-RIIa, FcRII-a, CD32, FCGR2A, FCG2, FCGR2A1, IGFR2, CD32a, FCG2A

Domain: 30-218aa
Sequence:
SADSQAAAPP KAVLKLEPPW INVLQEDSVT LTCQGARSPE SDSIQWFHNG NLIPTHTQPS YRFKANNNDS GEYTCQTGQT SLSDPVHLTV LSEWLVLQTP HLEFQEGETI MLRCHSWKDK PLVKVTFFQN GKSQKFSRLD PTFSIPQANH SHSGDYHCTG NIGYTLFSSK PVTITVQVPS MGSSSPMGI

UniProt No.: P12318
NCBI Accession No.: AAA35827.1

Molecular Weight: 21.7kDa (195aa)
Concentration: 1mg/ml (determined by Absorbance at 280nm)
Formulation: Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Purity: > 90% by SDS-PAGE
Bioactivity: Measured by its binding ability in a functional ELISA with Human IgG (CAT# ATGP4094).
Endotoxin: < 1 EU per 1ug of protein (determined by LAL method)
Endotoxin: His-Tag
Storage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.