Fc gamma RIIB/CD32b human recombinant protein

Background:
Fc gamma RIIB/CD32b, also known as FCGR2B, belongs to the Ig superfamily. It is a low-affinity receptor for the Fc region of immunoglobulins gamma that are complexed or aggregated. It participates in various effector and regulatory functions, such as phagocytosis of immune complexes and modulation of antibody production by B cells. Fc gamma RIIB/CD32b is expressed on B cells, monocytes, dendritic cells, neutrophils, mast cells, and basophils. When Fc gamma RIIB is ligated, it inhibits B cell expansion, plasma cell differentiation, production of autoimmune rheumatoid factors, and down-regulates TLR4 with reduced LPS responsiveness. Recombinant human Fc gamma RIIB/CD32b, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Alternative Names:
Low affinity immunoglobulin gamma Fc region receptor II-b isoform3, IgG Fc receptor II-b, CDw32, Fc-gamma RII-b, Fc-gamma-RIIb, FcRII-b, CD32, FCGR2B, FCG2, IGFR2, Fc fragment of IgG, low affinity II, low affinity IIb, Low affinity immunoglobulin gamma Fc region receptor II-c, IgG Fc receptor II-c, Fc-gamma RII-c, Fc fragment of IgG receptor IIb, Fc-gamma-RIIc, Fc gamma RIIb, CD32B, FcgammaRIIb, FCGR2, FCGR2C, FcGRIIB, FcRII-c

Domain: 46-217aa
Sequence:
APPKAVLKLE PQWINVLQED SVTLTCRGTH SPESDSIQWF HNGNLIPTHT QPSYRFKANN NDSGEYTCQT GQTSLSDPVH LTVLSEWLVL QTPHLEFQEG ETIVLRCHSW KDKPLVKVTF FQNGKSKKFS RSDPNFSIPQ ANHSHSGDYH CTGNIGYTLY SSKPVTITVQ AP

UniProt No.: P31994
NCBI Accession No.: NP_001002274.1

Molecular Weight: 20.1kDa (178aa)
Concentration: 1mg/ml (determined by Absorbance at 280nm)
Formulation: Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Purity: > 95% by SDS-PAGE
Bioactivity: Measured by its binding ability in a functional ELISA with Human IgG1 Fc (CAT# ATGP4094).
Endotoxin: < 1 EU per 1ug of protein (determined by LAL method)
Endotoxin: His-Tag
Storage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.