Ferritin Light Chain polyclonal, anti-human, mouse, rat

Background:
Mammalian ferritins consist of 24 subunits made up of two types of polypeptide chains, ferritin heavy chain and ferritin light chain, which each have unique functions. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of Fe (II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe (III). The most prominent role of mammalian ferritins is to provide iron-buffering capacity to cells. In addition to iron buffering, heavy chain ferritin is also involved in the regulation of thymidine biosynthesis via increased expression of cytoplasmic serine hydroxymethyltransferase, which is a limiting factor in thymidylate synthesis in MCF-7 cells. Light chain ferritin is involved in cataracts by at least two mechanisms, hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed, and oxidative stress, an important factor in the development of ageing-related cataracts. The gene encoding human ferritin heavy chain maps to chromosome 11q13 and the human ferritin light chain gene maps to chromosome 19q13.3-q13.4.

Alternative Name:
Ferritin L subunit, FTL, Ferritin L chain, Ferritin L subunit, Ferritin light chain, Ferritin light polypeptide, ferritin light polypeptide like 3, FRIL, FTL, LFTD, NBIA 3, NBIA3,

Application Dilution:
WB:1:1,000-1:5,000
IHC:1:100-1:500
ICC:1:100-1:200

Specificity: Ferritin Light Chain polyclonal antibody detects endogenous levels of Ferritin Light Chain protein.

Immunogen:
Recombinant protein

MW: 54 kDa

Swiss-Prot: P02792(Human) P29391(Mouse) P02793(Rat)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.