FGF-21 (clone FG224-7), anti-human, mouse

Decription:
FGF-21 stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of bKlotho.

Synonyms: Fibroblast Growth Factor 21

Clone: FG224-7

Isotype: Rat IgG1 kappa

Immunogen: Recombinant mouse FGF-21.

Reactivity: human, mouse

Specificity: Recognizes human and mouse FGF-21. Detects a band of ~25kDa by Western blot.

Form: Purified, in 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.

Applications:
ELISA: (direct or indirect: 1:2'000-1:5'000)
Western blot: (1:2'000-1:5'000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature).

Working Dilution: Optimal conditions must be determined individually for each application.

Concentration: 1mg/ml

Storage: 2-8C immediate use, or -20C (aliquote)