FGF-21 polyclonal, anti-human
€305.00
In stock
SKU
IMD-11180
Polyclonal Antibody against Human FGF-21
Application: ELISA
Host: Rabbit
INTRODUCTION
Fibroblast growth factor 21 (FGF-21) is a novel protein that has been implicated in the regulation of lipid and glucose metabolism under fasting and ketotic conditions [1,2]. In murine models, FGF-21 is predominantly expressed in liver, but also expressed in adipose tissue and pancreatic β-cells [3,4]. FGF-21 stimulates glucose uptake in adipocytes. It also protects animals from diet-induced obesity when overexpressed in transgenic mice and lowers blood glucose and triglyceride levels when administered to diabetic rodents [5]. When administered daily for 6 weeks to diabetic rhesus monkeys, FGF-21 caused a dramatic decline in fasting plasma glucose, fructosamine, triglycerides, insulin and glucagon [6]. Furthermore, elevated plasma FGF-21 concentrations in humans appear to be related to the presence of hepatic and peripheral insulin resistance [7].
IMMUNOGEN: Recombinant full-length human FGF-21 expressed in E.coli.
SPECIFICITY: The antibody detects circular human FGF-21.
FORMULATION: Liquid in phosphate-buffered saline (PBS).
STORAGE:
Store at –20°C for less than one week. For long-term STORAGE: , aliquot and freeze at -70°C. Avoid repeated freeze/defrost cycles.
APPLICATION/ USAGE
ELISA - The antibody can be used at 2 μg /mL with the appropriate secondary reagents to detect human FGF21.
PURIFICATION: Antigen affinity-purified
REFERENCES
[1] Kharitonenkov A, Shiyanova TL, et al. (2005) J Clin Invest; 115: 1627– 1635
[2] Badman MK, Pissios P, et al. (2007) Cell Metab; 5: 426– 437
[3] Nishimura T, Nakatake Y, et al. (2000) Biochim Biophys Acta; 1492: 203– 206
[4] Kurosu H, Choi M, et al. (2007) J Biol Chem; 282: 26687– 26695
[5] Kharitonenkov A, Shiyanova TL, et al. (2005) J. Clin. Invest. 115: 1627–35.
[6] Kharitonenkov A, Wroblewski VJ, et al. (2007) Endocrinology;148:774-81
[7] Chavez AO, Molina-Carrion M, et al. (2009) Diabetes Care; 32:1542-6.
PUBLICATIONS CITING THIS PRODUCT
Ye D, Li H, Wang Y, Jia W, Zhou J, Fan J, Man K, Lo C, Wong C, Wang Y, Lam KS. Circulating fibroblast growth factor 21 is a sensitive biomarker for severe ischemia/reperfusion injury in patients with liver transplantation. Scientific reports. 2016 Jan 25;6(1):1-9.
Application: ELISA
Host: Rabbit
INTRODUCTION
Fibroblast growth factor 21 (FGF-21) is a novel protein that has been implicated in the regulation of lipid and glucose metabolism under fasting and ketotic conditions [1,2]. In murine models, FGF-21 is predominantly expressed in liver, but also expressed in adipose tissue and pancreatic β-cells [3,4]. FGF-21 stimulates glucose uptake in adipocytes. It also protects animals from diet-induced obesity when overexpressed in transgenic mice and lowers blood glucose and triglyceride levels when administered to diabetic rodents [5]. When administered daily for 6 weeks to diabetic rhesus monkeys, FGF-21 caused a dramatic decline in fasting plasma glucose, fructosamine, triglycerides, insulin and glucagon [6]. Furthermore, elevated plasma FGF-21 concentrations in humans appear to be related to the presence of hepatic and peripheral insulin resistance [7].
IMMUNOGEN: Recombinant full-length human FGF-21 expressed in E.coli.
SPECIFICITY: The antibody detects circular human FGF-21.
FORMULATION: Liquid in phosphate-buffered saline (PBS).
STORAGE:
Store at –20°C for less than one week. For long-term STORAGE: , aliquot and freeze at -70°C. Avoid repeated freeze/defrost cycles.
APPLICATION/ USAGE
ELISA - The antibody can be used at 2 μg /mL with the appropriate secondary reagents to detect human FGF21.
PURIFICATION: Antigen affinity-purified
REFERENCES
[1] Kharitonenkov A, Shiyanova TL, et al. (2005) J Clin Invest; 115: 1627– 1635
[2] Badman MK, Pissios P, et al. (2007) Cell Metab; 5: 426– 437
[3] Nishimura T, Nakatake Y, et al. (2000) Biochim Biophys Acta; 1492: 203– 206
[4] Kurosu H, Choi M, et al. (2007) J Biol Chem; 282: 26687– 26695
[5] Kharitonenkov A, Shiyanova TL, et al. (2005) J. Clin. Invest. 115: 1627–35.
[6] Kharitonenkov A, Wroblewski VJ, et al. (2007) Endocrinology;148:774-81
[7] Chavez AO, Molina-Carrion M, et al. (2009) Diabetes Care; 32:1542-6.
PUBLICATIONS CITING THIS PRODUCT
Ye D, Li H, Wang Y, Jia W, Zhou J, Fan J, Man K, Lo C, Wong C, Wang Y, Lam KS. Circulating fibroblast growth factor 21 is a sensitive biomarker for severe ischemia/reperfusion injury in patients with liver transplantation. Scientific reports. 2016 Jan 25;6(1):1-9.
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