FHL2 polyclonal, anti-human, mouse, rat

Background:
The four-and-a-half-LIM domain (FHL) proteins include FHL-1 (SLIM1), FHL-2 (SLIM3), FHL-3 (SLIM2) and FHL-4. The signature "half-domain", a single zinc finger domain located in the N-terminal region, differentiates FHLs from other LIM-only proteins, which have numbers of zinc fingers. Specific combinations of FHL proteins elicit selective activation of both CREB and CREM. Skeletal and cardiac muscle express FHL-1 in high levels as compared to the low level of expression in smooth muscle of the colon, small intestine and prostate. FHL-1 localizes to the cytosol of myoblasts, myotubes, and differentiated myocytes. FHL-2 is also located in cardiac and skeletal muscle, as well as in placenta and ovary tissues. FHL-3 is found in skeletal muscle, but absent in cardiac muscle. FHL-4 is expressed exclusively by the seminiferous epithelium of the testis, which suggests that FHL-4 is involved in spermatogenesis. The genetic loci for FHLs vary considerably despite similiar amino acid sequences among the FHL group.

Alternative Name:
FHL-2, SLIM-3, FHL2, DRAL, SLIM3, AAG 11, AAG11, Aging associated gene 11, Down regulated in rhabdomyosarcoma LIM protein, Downregulated in rhabdomyosarcoma LIM protein, DRAL, FHL 2, FHL-2, Fhl2, FHL2 protein, FHL2, Four and a half LIM domain protein 2, Four and a half LIM domains 2, Four and a half LIM domains protein 2, KIAA0990, LIM domain protein DRAL, Skeletal muscle LIM protein 3, Skeletal muscle LIM-protein 3, SLIM 3, SLIM-3, SLIM3,

Application Dilution:
WB:1:500-1:2,000
IHC:1:50-1:200
IP:1:10-1:50

Specificity: FHL2 polyclonal antibody detects endogenous levels of FHL2 protein.

Immunogen:
Recombinant protein within C-terminal human FHL2.

MW: 32 kDa

Swiss-Prot: Q14192(Human) O70433(Mouse) O35115(Rat)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.