FHOD1 polyclonal, anti-human

Background:
Formins include several families of proteins that regulate actin cytoskeletal dynamics via two conserved formin homology domains, FH1 and FH2. The FH1 region contains poly-proline stretches that promote interactions with profilin. The FH2 domain, located C-terminally to the FH1 domain, is highly conserved in formin proteins and possesses actin nucleation and polymerization activities. Through cooperation of FH1 and FH2, formins construct actin-based structures comprising linear, unbranched filaments that are used in stress fibers, actin cables, microspikes, and contractile rings. Several mammalian formins, including mDia1, FRL, and formin homology domain protein 1 (FHOD1) are inhibited through an intramolecular interaction between the C-terminal Dia autoregulatory domain (DAD) and its recognition region at the N-terminus. In FHOD1, this autoinhibitory interaction is disrupted through phosphorylation of Ser-1131, Ser-1137, and Thr-1141 by ROCK. Subsequent FHOD1 activation leads to stress fiber formation. In endothelial cells, thrombin activates this ROCK pathway, leading to FHOD1-mediated stress fiber formation.

Background References
Westendorf, J.J. (2001) J Biol Chem. 276:46453.
Takeya, R. & Sumimoto, H. (2003) J Cell Sci. 116:4567.
Takeya, R. et al. (2008) EMBOJ 27:618.

Immunogen: FHOD1 recombinant protein that included the full length human FHOD1 sequence. This sequence has 50% homology to human FHOD3, and low homology to other formin family members.

Specificity: The antibody detects a 140 kDa* protein corresponding to the apparent molecular mass of FHOD1 on SDS-PAGE immunoblots of human K562 and Jurkat, and has weak reactivity to FHOD1 in mouse C2C12.

Buffer/Storage:
Mouse polyclonal antibody is supplied in 100μl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

Product References:
Truong, D. et al. (2013) Cell Microbiol. 15(12):2051. WB, ICC: HeLa transfection
Staus, D.P. et al. (2011) Arterioscler Thromb Vasc Biol. 31(2):360. WB: mouse, smooth muscle, siRNA