Fibrinogen polyclonal HRP, anti-rat

Quantity: 200 µl

Background:
The reactivity of the antiserum is restricted to fibrinogen. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against normal rat plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified fibrinogen. No reaction is obtained with any other plasma protein component or serum. However, the antiserum may also react with fibrin monomers, circulating fibrinopeptides and fibrin degradation products. In enzyme-immunocytochemical and immunohistochemical techniques for the detection of fibrinogen at the cellular and subcellular level in appropriately treated cell and tissue substrates; as detection reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA, Western blotting).This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:250; in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:4,000.

Isotype: Goat Polyclonal

Source: Fibrinogen (clotting factor I) is a heat labile beta glycoprotein present in plasma. It is the precursor of fibrin, which is the key protein constituting the network of the blood clot. Thrombin converts fibrinogen to fibrin by limited proteolysis. Fibrin monomers polymerize to fibrin which is stabilized by cross-linking. Fibrinogen is isolated from fresh plasma after removing prothrombin.Freund's complete adjuvant is used in the first step of the immunization procedure.

Form: Horseradish peroxidase-coupled purified hyperimmune IgG, in phosphate buffered saline (PBS, pH 7.2)

Specificity:
Horseradish peroxidase-conjugated IgG fraction of polyclonal Goat anti-Serum to Rat fibrinogen.

Reactivity: rat

Cross Reactivity:
The antiserum does not cross-react with any other component of Rat plasma. Inter-species crossreactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.

Applications: ELISA, Immunocytochemistry, Immunohistochemistry (paraffin), Dot blot, Immunoblotting

Storage: Store at 4C, or in small aliquots at -20C.