Fibrinopeptide E polyclonal, anti-human

Quantity: 200 µl

Background:
The reactivity of the antiserum is reacting with the fragment E as well as intact fibrinogen, fibrin monomers, fragment X and fragment Y. In immunoelectrophoresis, bidimensional electrophoresis and double radial immunodiffusion against normal human one single precipitin line is obtained. No reaction is obtained with any other plasma protein or normal serum. In immunoelectrophoresis use 2 ?l or equivalent against 120 ?l antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 ?l in a 3 mm diameter center well and 2 ?l serum samples (neat and serially diluted) in 2 mm diameter peripheral wells. In electroimmunodiffusion the amount of antiserum required in the gel is usually between 1 and 25 depending on the test arrangement

Isotype: Rabbit Polyclonal

Source: Fibrinogen (clotting factor I) is a heat labile beta glycoprotein (molecular weight 340,000) and consists of three pairs of chains bound by disulphide bonds. It is synthesized in hepatocytes under genetic control. It is the precursor of fibrin, which is the key protein constituting the network of the blood clot. Thrombin converts fibrinogen to fibrin by limited proteolysis, releasing the fibrinopeptides A and B (molecular weight 50,000-65,000) and forming fibrin monomers. Fibrin monomers polymerize to fibrin which is stabilized by cross-linking under the influence of factor XIII. The predominant gamma chain of normal fibrinogen (MW 50,000, with higher variants) has a low affinity for platelet binding. Fibrinogen is isolated from fresh plasma after removing prothrombin. Freund's complete adjuvant is used in the first step of the immunization procedure.

Form: Delipidated, heat inactivated, in PBS and 0.1% sodium azide.

Reactivity: human

Cross Reactivity:
The antiserum does not cross react with any other component of Human plasma. Inter-species crossreactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.

Applications: Immunoprecipitation

Storage: Store at 4C, or in small aliquots at -20C.