FITC (Fluorescein) (clone FLUO-1), anti-

Decription:
Rat monoclonal antibody to FITC

Synonyms: FITC, Fluorescein

Clone: FLUO-1

Isotype: Rat IgM kappa

Immunogen: Mouse IgG1 kappa labelled with FITC

Reactivity: FITC, Fluorescein Isothiocyanate

Specificity: Fluorescein Isothiocyanate

Form: Purified, in PBS, containing 0.1% Sodium Azide

Applications: As second antibody in immunoassays on a first FITC labelled antibody. Can be used for capture ELISA. In IFA, can clearly amplify the fluorescence intensity of a direct FITC staining, using the following system: first antibody-FITC + Mab FLUO-1-Biotin + Avidin-FITC.

Working Dilution: On FITC labelled BSA: 2 x 1010 M-1

Storage: 2-8C immediate use, or -20C (aliquot) extended storage