FITC (Fluorescein) (clone NI 239) HRP, anti-
€644.00
In stock
SKU
ARP-21-5083
Catalog Number: 21-5083
Size: 200 µg
Isotype: Mouse IgG1
Size: 200 µg
Isotype: Mouse IgG1
Background:
The reactivity of the antiserum is directed to the FITC molecule as tested in direct binding enzyme immunoassay, ELISA and immunoblotting. To enhance the specific signal obtained with a monoclonal antibody or a polyclonal second antibody conjugated to FITC. The phenomenon of a weak reaction of a monoclonal antibody is e.g. well known in different analysis of vital peripheral blood mononuclear cells in suspensions by the expression of surface markers. A similar situation exists in solid phase assay systems (ELISA, blotting, DIBA) when used for the identification and /or quantitative determination of minute amounts of soluble specific antigens or antibodies. The sensitivity of the FITC hapten-anti-hapten system makes it a valuable alternative to the biotinavidin system. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:100 to 1:500; in ELISA from 1:1,000 upwards; in Western blotting from 1:2,000 upwards. These data should be interpreted as general recommendations only.
Clone: NI 239
Isotype: Mouse IgG1
Source: Highly purified fluorescein isothiocyanate isomer 1.
Reactivity: Not applicable. This anti-serum has not been tested for cross-reactivity with other fluorescent compounds.
Specificity: Horseradish peroxidase conjugated purified monoclonal Mouse antibody to fluorescein isothiocyanate isomer 1
Form: Horseradish peroxidase conjugated purified mouse IgG1 lyophilized from a solution in phosphate buffered saline (pH 7.2). IgG concentration is 0.4 mg/ml. Peroxidase/IgG protein molar ratio (E/P) approximately 1.7. No foreign proteins added.
Applications: ELISA, Immunocytochemistry, Immunohistochemistry (paraffin), Dot blot, Immunoblotting
Storage: Store at 4C, or in small aliquots at -20C.
The reactivity of the antiserum is directed to the FITC molecule as tested in direct binding enzyme immunoassay, ELISA and immunoblotting. To enhance the specific signal obtained with a monoclonal antibody or a polyclonal second antibody conjugated to FITC. The phenomenon of a weak reaction of a monoclonal antibody is e.g. well known in different analysis of vital peripheral blood mononuclear cells in suspensions by the expression of surface markers. A similar situation exists in solid phase assay systems (ELISA, blotting, DIBA) when used for the identification and /or quantitative determination of minute amounts of soluble specific antigens or antibodies. The sensitivity of the FITC hapten-anti-hapten system makes it a valuable alternative to the biotinavidin system. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:100 to 1:500; in ELISA from 1:1,000 upwards; in Western blotting from 1:2,000 upwards. These data should be interpreted as general recommendations only.
Clone: NI 239
Isotype: Mouse IgG1
Source: Highly purified fluorescein isothiocyanate isomer 1.
Reactivity: Not applicable. This anti-serum has not been tested for cross-reactivity with other fluorescent compounds.
Specificity: Horseradish peroxidase conjugated purified monoclonal Mouse antibody to fluorescein isothiocyanate isomer 1
Form: Horseradish peroxidase conjugated purified mouse IgG1 lyophilized from a solution in phosphate buffered saline (pH 7.2). IgG concentration is 0.4 mg/ml. Peroxidase/IgG protein molar ratio (E/P) approximately 1.7. No foreign proteins added.
Applications: ELISA, Immunocytochemistry, Immunohistochemistry (paraffin), Dot blot, Immunoblotting
Storage: Store at 4C, or in small aliquots at -20C.
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