FLI1 polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
Friend leukemia integration 1 transcription factor, also known as Transcription factor ERGB or Proto-oncogene Fli-1 is a protein that in humans is encoded by the human FLI1 gene. The fli1 protooncogene encodes a member of the ETS family of winged helix-turn-helix transcription factors. This gene is mapped to 11q24.3. Fli-1 is activated through retroviral insertional mutagenesis in 90% of F-MuLV-induced erythroleukemias. The constitutive activation of fli-1 in erythroblasts leads to a dramatic shift in the Epo/Epo-R signal transduction pathway, blocking erythroid differentiation, activating the Ras pathway, and resulting in massive Epo-independent proliferation of erythroblasts. These results suggest that Fli-1 overexpression in erythroblasts alters their responsiveness to Epo and triggers abnormal proliferation by switching the signaling event(s) associated with terminal differentiation to proliferation.

Description:
Rabbit IgG polyclonal antibody for Friend leukemia integration 1 transcription factor (FLI1) detection. Tested with WB, IHC-P in Human, Mouse, Rat.

Synonyms:
ERGB transcription factor antibody, Ewing Sarcoma breakpoint region 2 antibody, EWSR 2 antibody, EWSR2 antibody, FLI 1 antibody, FLI 1 proto oncogene antibody, FLI1 antibody, FLI1 EWS fusion gene antibody, FLI1 proto oncogene antibody, FLI1_HUMAN antibody, Friend leukemia integration 1 transcription factor antibody, Friend leukemia virus integration 1 antibody, Proto-oncogene Fli-1 antibody, SIC 1 antibody, SIC1 antibody, Transcription factor ERGB antibody, Viral integration region FLI1 antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the N-terminus of human FLI1(54-73aa QQEWINQPVRVNVKREYDHM), identical to the related rat and mouse sequences.A synthetic peptide corresponding to a sequence at the N-terminus of human FLI1(54-73aa QQEWINQPVRVNVKREYDHM), identical to the related rat and mouse sequences.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, rat .
Predicted to work with: mouse.

Applications: WB, IHC-P

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.