FLIP (clone Dave-2), anti-human, mouse

Decription:
FLIP is an apoptosis regulator protein which functions as a crucial link between cell survival and cell death pathways in mammalian cells and acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. FLIP lacks enzymatic (caspase) activity. FLIP is highly expressed in skeletal muscle, pancreas, heart, kidney, placenta and peripheral blood leukocytes.

Synonyms: I-FLICE, CLARP, CASPER, Usurpin, CASH, FLAME-1

Clone: Dave-2

Isotype: Rat IgG2a

Immunogen: Recombinant human FLIP (aa 1-480).

Reactivity: human, mouse

Specificity: Recognizes an epitope (aa 1-200) present in both short (FLIPS) and long (FLIPL) splice variants of human and mouse FLIP.

Form: Purified, in PBS containing 10% glycerol and 0.02% sodium azide.

Applications:
Immunoprecipitation
Western Blot
Note: Not recommended for immunohistochemistry.

Working Dilution: Optimal conditions must be determined individually for each application.

Concentration: 1 mg/ml.

Storage: 2-8C immediate use, or -20C (aliquote)