Flotillin 1 polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
FLOT1(Flotillin 1), is a protein that in humans is encoded by the FLOT1 gene. The International Radiation Hybrid Mapping Consortium mapped the FLOT1 gene to chromosome 6. Bickel et al.(1997) found that mouse Flot1 behaves as a resident integral membrane protein of caveolae. It consistently copurified with Flot2 and with caveolin-1 in the purification of caveolin-rich membranes. Hazarika et al.(1999) found that stable transfection of Flot1, which they called ESA/flotillin-2, in COS-1 cells induced filopodia formation and changed the epithelial morphology to that of neuronal cells. Santamaria et al.(2005) found that prostate tumor overexpressed gene-1 interacted with flotillin-1 in detergent-insoluble membrane fractions. Flotillin-1 colocalized with PTOV1 at the plasma membrane and in the nucleus, and it entered the nucleus concomitant with PTOV1 shortly before initiation of S phase.

Description:
Rabbit IgG polyclonal antibody for Flotillin-1 (FLOT1) detection. Tested with WB in Human, Mouse, Rat.

Synonyms:
FLOT 1 antibody, FLOT1 antibody, FLOT1_HUMAN antibody, Flotillin-1 antibody, Flotillin1 antibody, Integral membrane component of caveolae antibody, Reggie 2 antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence in the middle region of humanFlotillin 1(219-234aa KKAAYDIEVNTRRAQA), different from the related rat and mouse sequences by one amino acid.A synthetic peptide corresponding to a sequence in the middle region of humanFlotillin 1(219-234aa KKAAYDIEVNTRRAQA), different from the related rat and mouse sequences by one amino acid.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, rat .
Predicted to work with: mouse.

Applications: WB

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.