Flotillin-2 (R144) polyclonal, anti-human, mouse, rat

Background:
Flotillins belong to a family of lipid raft-associated integral membrane proteins that carry a evolutionarily conserved domain called the prohibitin homology domain (PHB). Flotillin members are ubiquitously expressed and located to non-caveolar microdomains (lipid rafts) on the cell plasma membrane where they support signal transduction and regulate lipid raft motility and localization. Two flotillin members have been described, flotillin-1 and flotillin-2. In addition to its colocalization with lipid rafts on the plasma membrane, flotillin-1 also has been found at compartments of the endocytic and autophagosomal pathways, such as recycling/late endosomes, the Golgi complex, as well as the nucleus. Flotillin-2 is mainly localized to the plasma membrane and is prevalent in cell-cell contact sites. However, overexpressed flotillin-2 has also been found in the late endosome. Both flotillin-1 and flotillin-2 are commonly used as lipid raft associated markers.

Alternative Name:
Flotillin-2, Epidermal surface antigen, ESA, Membrane component chromosome 17 surface marker 1, ESA1, M17S1, FLOT2

Application Dilution:
WB: 1:500~1:1000

Specificity: Flotillin-2 (R144) polyclonal antibody detects endogenous levels of Flotillin-2 protein.

Immunogen:
Synthetic peptide, corresponding to amino acids 360-418 of Human TFDP1.

MW: ~ 49 kDa

Swiss-Prot: Q14254

Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

For research use only, not for use in diagnostic procedure.