Flt3 ligand/FLT3LG human recombinant protein

Background:
FLT3LG, also known as fms-related tyrosine kinase 3 ligand, is an alpha-cytokine that promotes the differentiation of multiple hematopoietic cell lineages. This protein acts as a growth factor that increases the number of immune cells by activating the hematopoietic progenitors. It is crucial for steady-state pDC and cDC development. It controls the development of DCs and is particularly important for plasmacytoid DCs and CD8- positive classical DCs and their CD103-positive tissue counterparts. Additionally, FLT3LG synergizes with IL-3, GM-CSF, and SCF to promote the mobilization and myeloid differentiation of hematopoietic stem cells. It cooperates with IL-2, IL-6, IL-7, and IL-15 to induce NK cell development and with IL-3, IL-7, and IL-11 to induce terminal B cell maturation. Animal studies show that it reduces the severity of experimentally induced allergic inflammation. Recombinant human FLT3LG, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Alternative Names:
Fms-related tyrosine kinase 3 ligand isoform 1, Fms-related tyrosine kinase 3 ligand, FLT3LG, FL, FLG3L, FLT3L, IMD125, Flt3 ligand, SL cytokine

Domain: 27-184aa
Sequence:
TQDCSFQHSP ISSDFAVKIR ELSDYLLQDY PVTVASNLQD EELCGGLWRL VLAQRWMERL KTVAGSKMQG LLERVNTEIH FVTKCAFQPP PSCLRFVQTN ISRLLQETSE QLVALKPWIT RQNFSRCLEL QCQPDSSTLP PPWSPRPLEA TAPTAPQP

UniProt No.: P49771
NCBI Accession No.: NP_001450

Molecular Weight: 19kDa (166aa)
Concentration: 0.5mg/ml (determined by absorbance at 280nm)
Formulation: Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Purity: > 95% by SDS-PAGE
Bioactivity: Measured in a cell proliferation assay using OCI-AML5 acute myeloid leukemia cells. The ED50 range ≤ 5 ng/ml.
Endotoxin: < 1 EU per 1ug of protein (determined by LAL method)
Endotoxin: His-Tag