Free Testosterone (F-TESTO) Detection Kit

Use: Used to detect the concentration of free testosterone (F-TESTO) in samples such as serum, plasma, and cell culture supernatant.Specificity: The kit uses enzyme-linked immunosorbent assay. Biotin-labeled free testosterone (F-TESTO) and purified anti-free testosterone (F-TESTO) antibodies are coated on microplates. In the competitive inhibition reaction, a certain amount of solid-phase antibodies inhibit the competitive reaction with biotin-labeled free testosterone (F-TESTO) and non-labeled antigens (calibrators or specimens). The binding amount of the antibody and biotin-labeled free testosterone (F-TESTO) is inhibited by the amount of non-labeled antigen. The more non-labeled antigen, the less the antibody and biotin-labeled free testosterone (F-TESTO) bind, and vice versa. After the reaction is balanced, a solid-phase antibody-biotinylated free testosterone (F-TESTO) is formed, and then enzyme-labeled avidin is added to form a solid-phase antibody-biotinylated free testosterone (F-TESTO)-enzyme-avidin complex. After adding substrate for color development, the absorbance (OD value) is measured at a wavelength of 450nm using an enzyme reader. As the concentration of free testosterone (F-TESTO) increases, the OD value gradually decreases and shows a good linear relationship. This kit has the characteristics of high sensitivity, strong specificity, good repeatability, simple operation, and rapidity, and has reliable detection performance for the reduction or increase of free testosterone (F-TESTO) in the sample.

Storage Conditions
Store at 2-8°C.

Use: The kit is for research use only.