GAD65 polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
Glutamate decarboxylase or glutamic acid decarboxylase(GAD) is an enzyme that catalyzes the decarboxylation of glutamate to GABA and CO2. GAD65 is the isoform with molecular weight of 65 kDa. Karlsen et al.(1991) mapped the GAD2 gene to chromosome 10p13-p11.2 by in situ hybridization. Bu et al.(1992) isolated a cDNA corresponding to the GAD2 gene from a human brain cDNA library. The deduced 585-amino acid protein has a molecular mass of 65 kD. GAD65 is target of autoantibodies in people who later develop type 1 diabetes mellitus or latent autoimmune diabetes.

Description:
Rabbit IgG polyclonal antibody for Glutamate decarboxylase 2 (GAD2) detection. Tested with WB, IHC-P in Human, Mouse, Rat.

Synonyms:
65 kDa glutamic acid decarboxylase antibody, DCE 2 antibody, DCE2 antibody, DCE2_HUMAN antibody, GAD 2 antibody, GAD 65 antibody, GAD-2 antibody, GAD-65 antibody, Gad2 antibody, Glutamate decarboxylase 2(pancreas) antibody, Glutamate Decarboxylase 2(pancreatic islets and brain 65kDa) antibody, Glutamate decarboxylase 2 antibody, Glutamate Decarboxylase 65 antibody, Glutamate decarboxylase 65 kDa isoform antibody, Glutamic Acid Decarboxylase 2 antibody, Glutamic Acid Decarboxylase 65 antibody, MGC161605 antibody, MGC161607 antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the N-terminus of human GAD65(21-37aa ENPGTARAWCQVAQKFT), identical to the related mouse and rat sequences.A synthetic peptide corresponding to a sequence at the N-terminus of human GAD65(21-37aa ENPGTARAWCQVAQKFT), identical to the related mouse and rat sequences.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, rat .
Predicted to work with: mouse.

Applications: WB, IHC-P

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.